Microscopy of vertical section of tulip stem

I bought my wife some tulip cut flowers last week – an ideal place to obtain a short section of stem. The biggest problem was finding a fresh sharp blade to cut it. Ideally, a new razor blade would do the job but I did not have one to hand!

An excellent resource which discusses what can be found in such sections can be read at http://microscopy-uk.org.uk/mag/indexmag.html?http://microscopy-uk.org.uk/mag/artfeb04/wdstem.html

Compare these pictures below to those from a horizontal section taken through the same plant stem today http://roslistonastronomy.uk/horizontal-section-tulip-stem-180318

In particular, see the image that author has published on that page of the spiral reinforcing structures in the vascular bundles of the stem – you can see these spiral reinforcing structures, cell walls, chloroplasts in my images below – although there are also linear artifacts from my cuts/folding tissue as I cut (basically it goes crinkly – although I floated the section on a drop of water on the slide to try and smooth this out. It is for this reason that I have not published any images of the Helicon Focus 3D depth maps – as the crinkling dominates the depth maps).

Andy

Tulip stem panorama 3176×5453 pixels below, 32x objective, bright field:

Detail of spiral supporting structure in vascular bundles x53 objective bright field:

Detail of cell wall boundary around edge of cell:

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