Microscopy open water trawl net samples from Branston Water Park collected 20/2/2018 viewed 22/2/2018

The following images are from the sample that Damian collected 20/2/2018 on our walk to Branston Water Park viewed through the Zeiss IM microscope.

I had kept the sample fed with sugar dissolved in water over last 2 days and in sunlight and exposed to oxygen (opened top bottle).

All images with Bresser Mikrocam 5.0 5MP camera.

The black circles are bubbles.

Specimens killed with 10% formalin (20ml poured into 2 litre bottle) and then filtered with coffee filter to concentrate the specimen. Specimen mounted using water-based mountant.

I am particularly proud of the first photo. Darkfield achieved using Ph2 Phase annulus on Zeiss IM microscope with x10 objective and then I used GIMP to darken background a little (I used curves).

Andy

Darkfield image x10 objective Phase 2 annulus:

Same field as above, bright field, x10 objective:

Following images are all brightfield, x2.7 objective:

 

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