The sample I took from the stream between Netherstowe School in Lichfield and adjacent allottments on 18/2/2018 has been kept in a jar and fed with a spoonful of sugar dissolved in warm water each day (to keep bacteria growing – these are bottom of food chain for larger predatory protozoa). I keep top open to allow oxygen in. The jars were kept next to our french doors to allow light to shine in each day.
Today I used phase contrast on the Zeiss IM microscope to view these images – so images and video below are either in bright field (without phase contrast) or Phase contrast using annulus Ph1 on the Zeiss. All images and video are using x32 long working distance objective and taken with Bresser 5MP Mikrocam camera.
Phase contrast images, x32 objective – show bacteria and a protozoal organism:
Bright field (first) and Phase contrast (Ph1) image (second) of same field to compare:
Video of sample x32 objective – this is brightfield not phase contrast: