I prepared a second slide, this time stained with H&E stain to show up nuclear and cytoplasmic material – as is common with bacterial cultures, all I tend to see is a lot of nuclear material.
x20 and x32 objectives, x1.0-x2.0 Optovar magnifier.
Fixed, stained slide.
I think that almost all the little dots are bacteria!….But don’t worry, this is a highly concentrated sample in a centrifuge.
See also associated post regarding phase contrast microscopy on same sample of pond water from Beacon Park:
Photographs of H&E stained slide – some of the photos are of dark microscopy achieved using phase annulus and phase objectives on H&E stained sections: