Microscopy from sample water pond at Beacon Park, Lichfield, 8/4/2020 – H&E staining

I prepared a second slide, this time stained with H&E stain to show up nuclear and cytoplasmic material – as is common with bacterial cultures, all I tend to see is a lot of nuclear material.

x20 and x32 objectives, x1.0-x2.0 Optovar magnifier.

Fixed, stained slide.

I think that almost all the little dots are bacteria!….But don’t worry, this is a highly concentrated sample in a centrifuge.

See also associated post regarding phase contrast microscopy on same sample of pond water from Beacon Park:

Microscopy from sample water pond at Beacon Park, Lichfield, 8/4/2020 – Phase Contrast

Andy

Photographs of H&E stained slide – some of the photos are of dark microscopy achieved using phase annulus and phase objectives on H&E stained sections:

        

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