Helicon Focus 6.8.0 – 3D modelling of microscopic images

I am very excited!

These images are amazing. My first attempts to produce 3D images from microscopic images on my Zeiss IM microscope. This software takes multiple images taken at different focus levels and combines them into a 3D model.

Tonight, I took a commercial stained slide of Volvox (pond water organism – ball of cells) and took a series of 7 photos and produced the 3D model frames of which can be seen below that. Following that, are photos and models of 4x objective view of stained flatworm.

Andy

Still photo – if you look carefully you can actually see that the central Volvox has collapsed centrally from its original ball in life during the fixing process:

The following is an image created from the seven images combined together to use the best focused parts of each image – this removes as far as possible the effect of depth of parts of the image on the slide to bring out as much detail as possible:

Frames from 3D model – this shows the central collapse and the different heights of different parts of the sample – down a microscope it looks like it is flat but the model shows that this is not really the case:

Using zoom function on 3D model – this blew my mind – the ability to zoom in to model in Helicon – however viewers must understand that the programme an combine detail from the images but not create detail where it is not present – so this image shows contours well but there is no individual cellular detail in this image as it was missing in the original photos:

3D depth map of the model:

Helicon combined image of one end of a flatworm (12 original images at different focus levels):

 

3D models:

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