The following images are taken on the Zeiss IM microscope using epi-illumination with the Zeiss 46 63 01 – 9901 filter cube from which I removed the filters. They show the effect of the coloured semi-silvered mirrors that remain in situ – these are required to direct the light and without them the microscope would not provide epi-illumination. Perhaps I can exchange them for non-pigmented mirrors in future?
Human-skin-commercial-slide-x20-obj-Kholer-illum-red-mirror-190218I.png – comparison image to above – same field of view – shows that epi-illumination is low contrast compared to transmitted light, particularly on this type of specimen. I have read similar in an article on Micscape website:
Comparison image of Aspergillus via transmitted light:
The following picture is a photo composite created from a video that goes through focus using Helicon Focus stacking software – this allows the best bits of focus at different levels to be combined: