- Zeiss IM microscope
- 20x brightfield objective
- Calibration slide
- One photo with no filter
- Second photo using plastic 32mm filter before condenser – this is matt black with crescent shaped hole cut into one side – I tried ones with various sized central obstructions and seemed to make little difference! This is a set of filters purchased from ebay this week for £12.
There seems to be a slight difference with features on slide put into some relief – but perhaps I am imagining it!
Andy
Zeiss-IM-microscope-20x-brightfield-objective-brightfield-no-filter-130517.bmp (below):
Zeiss-IM-microscope-20x-brightfield-objective-brightfield-crescent-shaped-darkfield-filter-130517.bmp (below):