Microscopy of diatom in water from canal @ Samuel Barlow @ Alvecote

Alan and Angella took me out for lunch to the Samuel Barlow @ Alvecote – a lovely little pub next to the canal. Before we left, we took a water sample from the canal. The sample proved to be relatively sterile – I suspect due to oil from the multiple boats in local marina.

Andy

On analysing the specimen, this diatom could be seen, viewed through my Zeiss IM35 microscope. I concentrated the specimen first using a centrifuge. Otherwise the following are live unstained views. Colours on the diatom are due to refraction as the organism’s shell is crenulated, refracting light, and has depth making it impossible to focus precisely upon it, worse when magnification increased.

 

Photo x20 objective:

Photo x32 objective:

Video (various objectives):

https://youtu.be/HMyUq8tKuuw

https://youtu.be/QveTzCODOSM

 

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