Pond, lake, stream and river water (fresh water) microscopy

Microscopy stagnant pool Hopwas Woods (next to canal) 17/9/2019

My god-son Laurence and I went for a walk next to the canal at Hopwas Woods near Lichfield today and took a water sample from a stagnant pool next to the canal.

Images below with 100x oil immersion lens (with immersion oil), Bresser MicroCam SP 5.1 camera.

Stained with H&E stain, heat fixation.

I used Registax 6 to stack video files and GIMP to stretch the histogram – applying astronomy imaging techniques to microscopy!

Andy

We found this organism attached to a piece of vegetation – note the multiple linear structures along edge of its body – video below shows us changing focus through body of the organism – multiple blue dots are bright/dead pixels on camera – Oh what I wouldn’t give for a COOLED microscope camera!

Diatom skeleton showing structure in skeleton:

Microscopy Kingsbury Water Park Angella, Alan, Andrew 9/8/2019

The three of us collected samples today from Kingsbury Water Park near M42 – weather was rather variable but we had great fun and shows what can be done with simple kit

Andy

Alan & Andrew at Kingsbury Water Park:

Feather, using Alan and Angella’s own ex-school binocular microscope:

Alan and Angella’s pictures of vegetative matter from pool under Leitz microscope with x10 and x20 objectives:

Phase contrast microscopy of sediment from bottom of pot pond 2/8/2019

It has been a long time since I last did some phase contrast microscopy with my Zeiss IM35 microscope so here is a quick study of some sediment from the bottom of my pot pond.

Andy

Algae – x10 objective, variety of Phase and illumination settings (all transilluminated – various aperture settings on illuminator and light intensity, and Phase 1/2):

Algae fond pot pond x32 obj x2 Optovar 020819:

Video:

Microscopy of water from Stowe Pool, Lichfield, 31/7/2019

This year, I haven’t had much opportunity to use my microscope.

My daughter Hannah and I collected a sample of water from Stowe Pool in Lichfield and I looked at it under my Zeiss IM35 microscope.

Andy

 

Photos:

x4 objective, x2 Optovar (equivalent x8 Objective):

Shows protozoa feeding on vegetative debris. See video below for movement of multiple protozoal organisms in this field of view.

x32 objective:

Ball of cells – cilia visible on each cell:

Cilia visible from corners of stack of cells, and below this diatom:

Algae:

Stack 4 cells with cilia at corners:

Photo x32 objective, x2 Optovar (equivalent to x64 Objective):

Detail of cell wall and contents at junction between two algae cells:

Algae fonds x32 Obj x2 Optovar:

Stack 4 cells with cilia at corners:

 

Video:

https://youtu.be/vqNVLIrg65E

 

Microscopy of diatom in water from canal @ Samuel Barlow @ Alvecote

Alan and Angella took me out for lunch to the Samuel Barlow @ Alvecote – a lovely little pub next to the canal. Before we left, we took a water sample from the canal. The sample proved to be relatively sterile – I suspect due to oil from the multiple boats in local marina.

Andy

On analysing the specimen, this diatom could be seen, viewed through my Zeiss IM35 microscope. I concentrated the specimen first using a centrifuge. Otherwise the following are live unstained views. Colours on the diatom are due to refraction as the organism’s shell is crenulated, refracting light, and has depth making it impossible to focus precisely upon it, worse when magnification increased.

 

Photo x20 objective:

Photo x32 objective:

Video (various objectives):

https://youtu.be/HMyUq8tKuuw

https://youtu.be/QveTzCODOSM

 

Sructures at edge of algae from pot pond

The following two photos show structures on edge of an algal strand from my pot pond – not sure if these are part of algae or other organisms which are using it as a platform to base themselves….

  • Leitz Laborlux 11 Microsocope
  • x40 objective
  • Bright field
  • DCM-310 Camera for Microscope.
  • ScopePhoto 64 bit software
  • GIMP2 post-processing.

Andy

Microscopy of sample from bottom of pot pond 11/11/2018

For last few months, I have been cultivating a “pond” in a large pot in my garden.

The following photos are taken from a sample from the bottom of this pond today, using my Leitz Laborlux 11 microscope and Bresser Microcam SP 5.1 camera, with x4, x10, x40 objectives.

The photos and video below are all based around highly magnified microscopy of the antennae/legs of a small 2-3mm crustacean I found in the sample. In particular, I focus on other animal life (single and multicellular) living on or around these structures.

Obj = microscope objective power.

Andy

Detail-crustacean-pot-pond-Leitz-Laborlux-11-x4-obj-111118:

See the small group of oval objects attached to the antenna on the right – I think this is a group of other organisms using the crustacean as a platform!

On the other two legs visible, note the nodular structure to the chiton exoskeleton. Plenty of hairs to be seen projecting from legs and antenna.

Those group of oval organisms are seen attached to the antenna at bottom of photo below (photo & video):

Antenna (below):

 

Detail-crustacean-pot-pond-Leitz-Laborlux-11-x10-obj-111118:

   Detail-crustacean-pot-pond-Leitz-Laborlux-11-x40-obj-111118:

These images show close ups of where the hairs arise from the chiton exoskeleton of the legs.

Rolling ball cells pot pond Leitz Laborlux 11 x10 obj 111118 (below):

Rolling ball cells pot pond Leitz Laborlux 11 x40 obj 111118 (photo and video):

Worm pot pond Leitz Laborlux 11 x4 obj 111118 (below, photos & video):

Worm pot pond Leitz Laborlux 11 x10 obj 111118 (below, photos & video):

 

Bright field vs. crossed polarisation views of microscope slide of material from LRO pot pond 28/5/2018

I used the centrifuge to concentrate down material from “open water” in the pot pond (i.e. not near edge or bottom or plants) and views the result in the LOMO Polam P-113 microscope, by bright field & crossed polarisation.

Note that such open water samples do not contain diatoms – which are famously birefringent.

Andy

Birefringement material pot pond slide 280518@1642, x20 objective:

Birefringent material slide from pot pond 280518@1703, x20 objective:

Insect on slide pot pond water 280518@1626 panorama, x20 objective:

Slide from pot pond: birefringent material shown in sequence as I moved the second polarised filter from 0-90 degrees so that the sample moved between crossed polarisation and bright field, 280518@1639 (below):

Slide from pot pond highly birefringent organisms brightfield x20 obj 280518@1635: