Unprocessed, unstained photos of section of fish fin from Sea Bass.
Black and yellow pigment is visible – as magnification increases it can be seen that the pigment is not neatly laid out but looks almost as though someone has thrown ink out of end of pen onto the fin! Pigment is produced by pigment-producing cells so this must reflect the distribution of those cells in the fin.
We had fish for tea! So here is a small piece of salmon meat (cooked) seen under Zeiss IM microscope bright-field with x4 and x32 objectives. Bresser MikrOkular camera.
x4 objective – the lines show divisions between bundles of muscle fibres. They go in the same direction as the muscle fibres are orientated in the same direction:
x4 objective – I think the muscle fibre bundles are facing more towards us in this image hence the change in shape of the dividing fibrous tissue lines:
x4 objective – torn end of muscle:
x32 objective – the end of the muscle sample – torn off from the fish this shows the broken end of the piece of cooked muscle:
Helicon Focus stack:
Helicon Focus 3D model from above stack:
x32 objective – the following photo shows striations across the fish muscle. I am not sure what this is, but it might be end of view of the muscle fibres or separation of fibres as a result of denaturisation from cooking process: