Flourescence microscopy equipment

Illuminator for epi-illumination during fluorescence microscopy with Zeiss IM/IM35 microscope

This is the illuminator I have obtained which fits on the epi-illumination port at the back of the microscope: Zeiss part 46 72 59 – 9901. This is the illuminator which is meant to be used with a mercury lamp for fluorescence microscopy.

It is quite a heavy bit of kit and like everything that fits on these microscopes made of metal and built like a tank!

The following link opens a manual which seems to work for this illuminator, although I am not sure whether it is exactly the right one:

Zeiss Illuminator 100 user manual (PDF file)

Andy

The next picture shows a small Allen head screw in the central depression on the bottom of the illuminator. Loosening this (does not need to be completely removed) allows the lamp and its base to be slipped easily out of the housing for examination +/- replacement:

The next pictures are of the lamp that is in the illuminator – this came with it when I purchased it from ebay:

 

I think this is a new version of the lamp above:

100W appears to be the correct rating for this illuminator as noted on the underside of the lamp base:

 

Fluorescence cubes for Zeiss IM and IM35 microscopes

I have added some posts re fluorescence microscopic images with my Zeiss IM microscope. This is very much early days but has been made possible by the purchase of a second hand (everything to do with this microscope is of necessity second hand as it is somewhat antique) fluorescence slider with two cubes on it – Zeiss item 46 63 01 – 9901.

I am not sure what the filters are that are on it but I have seen an advert for another IM microscope being sold with similar slider and there the filters are listed as:

Including 50w HBO (mercury) illuminator and 2 complete filter cubes that appear to be in excellent condition:

Cube#1: FITC. Excitation: 450-490nm. Dichroic Mirror: FT 510nm. Emission Spectra: Long Pass LP 520.

Cube#2: UV. Excitation: Band Pass 365nm. Dichroic Mirror: FT 395nm. Emission Spectra: Long Pass LP 397.

So perhaps my filters are the same – one thing I do need to do is check my epi-illuminator does not have mercury bulb in it – rather dangerous! Actually, if it does now then this raises a big question about how I can illuminate any fluorescent dyes or where I can get any fluorescent dyes from in the first place……but those are questions for another day!

The filters are somewhat loose in the cubes – so that is also something I need to sort out. I think the glue has gone loose. Considering that such filters are many hundreds pounds each, I am somewhat relunctant to try gluing them as I am concerned the glue will ruin them – I wonder if I can find some appropriately sized rubber/plastic washers.

Andy

Possible washers for fluorescence cubes: