Alan and Angella took me out for lunch to the Samuel Barlow @ Alvecote – a lovely little pub next to the canal. Before we left, we took a water sample from the canal. The sample proved to be relatively sterile – I suspect due to oil from the multiple boats in local marina.
On analysing the specimen, this diatom could be seen, viewed through my Zeiss IM35 microscope. I concentrated the specimen first using a centrifuge. Otherwise the following are live unstained views. Colours on the diatom are due to refraction as the organism’s shell is crenulated, refracting light, and has depth making it impossible to focus precisely upon it, worse when magnification increased.
This fungus was growing on the earth around the base of the plant in a plant pot in our kitchen in Lichfield. Interconnecting hypae can be seen but the stand-out difference between these photos and those of fungus on bread and cheese I have posted in past is the lack of spores.
Leitz Laborlux 11 microscope.
Magnification 10-100x objectives. The 100x objective was oil immersion and I could not find the proper oil so used extra virgin olive oil as my immersion oil!
Branching hyphae matrix:
x100 oil immersion objective, using virgin olive oil as immersion oil (as I could not find the proper immersion oil bottle!):
About a month ago, I placed a slide in my pot pond outside and the algae on the photos below are those that have grown in situ. The slide was in the top ten centimetres of the pot pond – where sunlight would hit the slide every day.
This has also been an opportunity to try out my QHY6 mono astronomical camera on my Zeiss IM microscope & compare photos taken with that camera with those taken with my trusty Bresser Mikrocam SP 5.1 camera – the main issue is the small sensor on the QHY6 gives highly magnified image & loss of detail as in monochrome.
For last few months, I have been cultivating a “pond” in a large pot in my garden.
The following photos are taken from a sample from the bottom of this pond today, using my Leitz Laborlux 11 microscope and Bresser Microcam SP 5.1 camera, with x4, x10, x40 objectives.
The photos and video below are all based around highly magnified microscopy of the antennae/legs of a small 2-3mm crustacean I found in the sample. In particular, I focus on other animal life (single and multicellular) living on or around these structures.
Elodea is a genus of 6 species of aquatic plants often called the waterweeds described as a genus in 1803. Elodea is native to North and South America and is also widely used as aquarium vegetation. It lives in fresh water (Wikipedia). Chloroplasts can move in all plants but are particularly visible in Elodea.
I used my Leitz Laborlux 11 microscope today to view a thin slice of Elodea leaf with a bright light from the side to stimulate movement.
Video of chloroplast movement in Elodea, Leitz Laborlux 11 microscope, 40x objective:
Video of chloroplast movement in Elodea, Leitz Laborlux 11 microscope, 100x objective:
In the next photo, look carefully – there are many tiny organelles visible apart from the more obvious chloroplasts: