Equipment for microscopy

Microscopy of sourdough starter

My daughter Hannah has made some sourdough – so I thought I’d take a look under the microscope.

Following photos – 32x objective, 2.5x Optovar, brightfield (so equivalent to 80x objective) – except last one which is Phase 2.

I am not sure whether circular bodies are air bubbles or yeast cells or something else….

Andy

Brightfield:

Phase 2:

Phase contrast microscopy of sediment from bottom of pot pond 2/8/2019

It has been a long time since I last did some phase contrast microscopy with my Zeiss IM35 microscope so here is a quick study of some sediment from the bottom of my pot pond.

Andy

Algae – x10 objective, variety of Phase and illumination settings (all transilluminated – various aperture settings on illuminator and light intensity, and Phase 1/2):

Algae fond pot pond x32 obj x2 Optovar 020819:

Video:

Microscopy of water from Stowe Pool, Lichfield, 31/7/2019

This year, I haven’t had much opportunity to use my microscope.

My daughter Hannah and I collected a sample of water from Stowe Pool in Lichfield and I looked at it under my Zeiss IM35 microscope.

Andy

 

Photos:

x4 objective, x2 Optovar (equivalent x8 Objective):

Shows protozoa feeding on vegetative debris. See video below for movement of multiple protozoal organisms in this field of view.

x32 objective:

Ball of cells – cilia visible on each cell:

Cilia visible from corners of stack of cells, and below this diatom:

Algae:

Stack 4 cells with cilia at corners:

Photo x32 objective, x2 Optovar (equivalent to x64 Objective):

Detail of cell wall and contents at junction between two algae cells:

Algae fonds x32 Obj x2 Optovar:

Stack 4 cells with cilia at corners:

 

Video:

https://youtu.be/vqNVLIrg65E

 

Microscopy of diatom in water from canal @ Samuel Barlow @ Alvecote

Alan and Angella took me out for lunch to the Samuel Barlow @ Alvecote – a lovely little pub next to the canal. Before we left, we took a water sample from the canal. The sample proved to be relatively sterile – I suspect due to oil from the multiple boats in local marina.

Andy

On analysing the specimen, this diatom could be seen, viewed through my Zeiss IM35 microscope. I concentrated the specimen first using a centrifuge. Otherwise the following are live unstained views. Colours on the diatom are due to refraction as the organism’s shell is crenulated, refracting light, and has depth making it impossible to focus precisely upon it, worse when magnification increased.

 

Photo x20 objective:

Photo x32 objective:

Video (various objectives):

https://youtu.be/HMyUq8tKuuw

https://youtu.be/QveTzCODOSM

 

Microscopy of fungus from plant pot in kitchen 9/12/2018 – Leitz Laborlux 11, Bresser Mikrocam SP5.1 camera, oil immersion objective

This fungus was growing on the earth around the base of the plant in a plant pot in our kitchen in Lichfield. Interconnecting hypae can be seen but the stand-out difference between these photos and those of fungus on bread and cheese I have posted in past is the lack of spores.

  • Leitz Laborlux 11 microscope.
  • Magnification 10-100x objectives. The 100x objective was oil immersion and I could not find the proper oil so used extra virgin olive oil as my immersion oil!

Andy

x10 objective:

SIngle hyphus:

Branching hyphae matrix:

x40 objective:

x100 oil immersion objective, using virgin olive oil as immersion oil (as I could not find the proper immersion oil bottle!):

Sructures at edge of algae from pot pond

The following two photos show structures on edge of an algal strand from my pot pond – not sure if these are part of algae or other organisms which are using it as a platform to base themselves….

  • Leitz Laborlux 11 Microsocope
  • x40 objective
  • Bright field
  • DCM-310 Camera for Microscope.
  • ScopePhoto 64 bit software
  • GIMP2 post-processing.

Andy

Algae growing on Pot Plant Slide 1/12/2018 – Zeiss IM microscope; QHY6 mono camera vs. Bresser Mikrocam SP5.1 colour camera

About a month ago, I placed a slide in my pot pond outside and the algae on the photos below are those that have grown in situ. The slide was in the top ten centimetres of the pot pond – where sunlight would hit the slide every day.

This has also been an opportunity to try out my QHY6 mono astronomical camera on my Zeiss IM microscope & compare photos taken with that camera with those taken with my trusty Bresser Mikrocam SP 5.1 camera – the main issue is the small sensor on the QHY6 gives highly magnified image & loss of detail as in monochrome.

Andy

x4 objective, QHY6 mono camera:

x4 objective, Bresser Mikrocam SP5.1 colour camera:

x32 objective, QHY6 mono camera:

x32 objective, Bresser Mikrocam SP5.1 colour camera:

Microscopy of sample from bottom of pot pond 11/11/2018

For last few months, I have been cultivating a “pond” in a large pot in my garden.

The following photos are taken from a sample from the bottom of this pond today, using my Leitz Laborlux 11 microscope and Bresser Microcam SP 5.1 camera, with x4, x10, x40 objectives.

The photos and video below are all based around highly magnified microscopy of the antennae/legs of a small 2-3mm crustacean I found in the sample. In particular, I focus on other animal life (single and multicellular) living on or around these structures.

Obj = microscope objective power.

Andy

Detail-crustacean-pot-pond-Leitz-Laborlux-11-x4-obj-111118:

See the small group of oval objects attached to the antenna on the right – I think this is a group of other organisms using the crustacean as a platform!

On the other two legs visible, note the nodular structure to the chiton exoskeleton. Plenty of hairs to be seen projecting from legs and antenna.

Those group of oval organisms are seen attached to the antenna at bottom of photo below (photo & video):

Antenna (below):

 

Detail-crustacean-pot-pond-Leitz-Laborlux-11-x10-obj-111118:

   Detail-crustacean-pot-pond-Leitz-Laborlux-11-x40-obj-111118:

These images show close ups of where the hairs arise from the chiton exoskeleton of the legs.

Rolling ball cells pot pond Leitz Laborlux 11 x10 obj 111118 (below):

Rolling ball cells pot pond Leitz Laborlux 11 x40 obj 111118 (photo and video):

Worm pot pond Leitz Laborlux 11 x4 obj 111118 (below, photos & video):

Worm pot pond Leitz Laborlux 11 x10 obj 111118 (below, photos & video):

 

Chloroplast movement in Elodea 11/11/2018

Elodea is a genus of 6 species of aquatic plants often called the waterweeds described as a genus in 1803. Elodea is native to North and South America and is also widely used as aquarium vegetation. It lives in fresh water (Wikipedia). Chloroplasts can move in all plants but are particularly visible in Elodea.

I used my Leitz Laborlux 11 microscope today to view a thin slice of Elodea leaf  with a bright light from the side to stimulate movement.

Andy

Video of chloroplast movement in Elodea, Leitz Laborlux 11 microscope, 40x objective:

 

Video of chloroplast movement in Elodea, Leitz Laborlux 11 microscope, 100x objective:

Photos:

x40 objective:

In the next photo, look carefully – there are many tiny organelles visible apart from the more obvious chloroplasts:

x100 objective: