Microscopy

Microscopy Kingsbury Water Park Angella, Alan, Andrew 9/8/2019

The three of us collected samples today from Kingsbury Water Park near M42 – weather was rather variable but we had great fun and shows what can be done with simple kit

Andy

Alan & Andrew at Kingsbury Water Park:

Feather, using Alan and Angella’s own ex-school binocular microscope:

Alan and Angella’s pictures of vegetative matter from pool under Leitz microscope with x10 and x20 objectives:

Microscopy of sourdough starter

My daughter Hannah has made some sourdough – so I thought I’d take a look under the microscope.

Following photos – 32x objective, 2.5x Optovar, brightfield (so equivalent to 80x objective) – except last one which is Phase 2.

I am not sure whether circular bodies are air bubbles or yeast cells or something else….

Andy

Brightfield:

Phase 2:

Phase contrast microscopy of sediment from bottom of pot pond 2/8/2019

It has been a long time since I last did some phase contrast microscopy with my Zeiss IM35 microscope so here is a quick study of some sediment from the bottom of my pot pond.

Andy

Algae – x10 objective, variety of Phase and illumination settings (all transilluminated – various aperture settings on illuminator and light intensity, and Phase 1/2):

Algae fond pot pond x32 obj x2 Optovar 020819:

Video:

Microscopy of water from Stowe Pool, Lichfield, 31/7/2019

This year, I haven’t had much opportunity to use my microscope.

My daughter Hannah and I collected a sample of water from Stowe Pool in Lichfield and I looked at it under my Zeiss IM35 microscope.

Andy

 

Photos:

x4 objective, x2 Optovar (equivalent x8 Objective):

Shows protozoa feeding on vegetative debris. See video below for movement of multiple protozoal organisms in this field of view.

x32 objective:

Ball of cells – cilia visible on each cell:

Cilia visible from corners of stack of cells, and below this diatom:

Algae:

Stack 4 cells with cilia at corners:

Photo x32 objective, x2 Optovar (equivalent to x64 Objective):

Detail of cell wall and contents at junction between two algae cells:

Algae fonds x32 Obj x2 Optovar:

Stack 4 cells with cilia at corners:

 

Video:

https://youtu.be/vqNVLIrg65E

 

Microscopy of diatom in water from canal @ Samuel Barlow @ Alvecote

Alan and Angella took me out for lunch to the Samuel Barlow @ Alvecote – a lovely little pub next to the canal. Before we left, we took a water sample from the canal. The sample proved to be relatively sterile – I suspect due to oil from the multiple boats in local marina.

Andy

On analysing the specimen, this diatom could be seen, viewed through my Zeiss IM35 microscope. I concentrated the specimen first using a centrifuge. Otherwise the following are live unstained views. Colours on the diatom are due to refraction as the organism’s shell is crenulated, refracting light, and has depth making it impossible to focus precisely upon it, worse when magnification increased.

 

Photo x20 objective:

Photo x32 objective:

Video (various objectives):

https://youtu.be/HMyUq8tKuuw

https://youtu.be/QveTzCODOSM

 

Sap from leaf 6/1/2019

Following are photos from sap coming out of cut end of house plant leaf.

Zeiss IM microscope.

No stain.

Andy

x32 objective, Optovar x1 intermediate lens:

x63 objective, Optovar x1 intermediate lens:

x63 objective, Optovar x2 intermediate lens, some sharpening with unsharp mask in GIMP2:

x63 objective, Optovar x2 intermediate lens, further contrast and brightness enhanced in GIMP2:

Microscopy of fungus from plant pot in kitchen 9/12/2018 – Leitz Laborlux 11, Bresser Mikrocam SP5.1 camera, oil immersion objective

This fungus was growing on the earth around the base of the plant in a plant pot in our kitchen in Lichfield. Interconnecting hypae can be seen but the stand-out difference between these photos and those of fungus on bread and cheese I have posted in past is the lack of spores.

  • Leitz Laborlux 11 microscope.
  • Magnification 10-100x objectives. The 100x objective was oil immersion and I could not find the proper oil so used extra virgin olive oil as my immersion oil!

Andy

x10 objective:

SIngle hyphus:

Branching hyphae matrix:

x40 objective:

x100 oil immersion objective, using virgin olive oil as immersion oil (as I could not find the proper immersion oil bottle!):