Alan and Angella took me out for lunch to the Samuel Barlow @ Alvecote – a lovely little pub next to the canal. Before we left, we took a water sample from the canal. The sample proved to be relatively sterile – I suspect due to oil from the multiple boats in local marina.
On analysing the specimen, this diatom could be seen, viewed through my Zeiss IM35 microscope. I concentrated the specimen first using a centrifuge. Otherwise the following are live unstained views. Colours on the diatom are due to refraction as the organism’s shell is crenulated, refracting light, and has depth making it impossible to focus precisely upon it, worse when magnification increased.
This fungus was growing on the earth around the base of the plant in a plant pot in our kitchen in Lichfield. Interconnecting hypae can be seen but the stand-out difference between these photos and those of fungus on bread and cheese I have posted in past is the lack of spores.
Leitz Laborlux 11 microscope.
Magnification 10-100x objectives. The 100x objective was oil immersion and I could not find the proper oil so used extra virgin olive oil as my immersion oil!
Branching hyphae matrix:
x100 oil immersion objective, using virgin olive oil as immersion oil (as I could not find the proper immersion oil bottle!):