Microscopy of culture of moss collected 7 days ago

This sample was collected from our garden 7 days ago and kept in an open jar of water.

The contents of the jar had separated into a top layer of moss floating on the top, an intermediate layer of very cloudy water and a bottom layer of debris on the bottom of the jar. I have tried to sample all three layers in the pictures below.

Andy

x20 objective bright field sample from bottom of jar – debris layer. This shows large numbers of bacteria.

x32 objective bright field bottom debris layer:

Moss 7 day culture bottom jar layer video x32 objective Phase I annulus:

 

x20 phase contrast I debris layer bottom jar:

x32 objective phase contrast I debris layer jar:

x20 objective phase contrast I cloudy liquid layer between debris on bottom and floating moss – I am not convinced that this is phase contrast even though I labelled it as such – looks like bright field to me now:

x20 bright field liquid layer between debris and moss – video:

 

x20 bright field one single moss plant from the floating moss on top of the jar. If you look carefully you can see hundreds of bacteria surrounding this plant:

 

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