Horizontal section Tulip stem with and without polarisation, bright field

To interpret these pictures see An excellent resource which discusses what can be found in such sections can be read at http://microscopy-uk.org.uk/mag/indexmag.html?http://microscopy-uk.org.uk/mag/artfeb04/wdstem.html

The following image comes from this resource and is labelled to show which structure is which (Walter Dioni, http://microscopy-uk.org.uk/mag/indexmag.html?http://microscopy-uk.org.uk/mag/artfeb04/wdstem.html):

ep = skin of the stem – col = colenchyma – par = cortical parenchyma (colenchyme + parenchyma form the cortex) –cb = cambium interfasciculaire – Paq.vasc. = package or vascular bundle formed by the phloem (ph) and the xylem (xy). Later it will be seen with more details – moelle, the central cylinder of parenchyma (the pith). Cambium plus pith form a central cylinder: the stele. The raphides are oxalate of calcium needles secreted by some cells. Here, the edges of the razor blades have cut an epithelial cell full of raphides throwing them on the cuted surface

Compare these photos today with those taken through a vertical section through this same plant stem http://roslistonastronomy.uk/vertical-section-tulip-stem-180318

I think the dark brown/black areas between cells in my photos below are vascular bundles seen end on, with the thick spiral supporting structure shown in the photos in my photos of the vertical section seen at the link above acting to block out light when seen end on. I can not identify raphides in the pictures below but I think I may have seen them in another post – see http://roslistonastronomy.uk/plant-stem-from-kitchen

Andy

My photos from today:

x10 objective, bright field:

x20 objective, bright field:

x32 objective, bright field:

x10 objective bright field crossed polarisation – varying rotation angle of one filter (below):

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