Damian processes Stowe Pool microscope slides for observing under Zeiss IM microscope with Andy

Damian came around this afternoon and took to the microscope like a pro!

He processed slides from a sample of pond water I collected yesterday and kept in my mini-aquarium (square glass vase of Ean Ean’s) overnight – producing live, heat fixed, heat fixed and H&E stained slides, which were then observed with x4, x10, x20, x32 and x40 objectives and photographs and video taken using the Mikrocam 9.0 camera on my Dell E4800 laptop.

Andy

Damian processing slides in my kitchen sink:

The slides Damian produced:

Micrometer eyepiece and calibration slide:

Microcamlab7 software screen photo grab with calibration slide with 20x objective:

The following photo was taken at the eyepiece using Samsung S7 through the LOMO micrometer eyepiece and with calibration slide in field of view:

Video from microscopy session, by Damian and Andy. Most of the video covers views of live slides, in direct and phase contrast transmitted light, using 30W www.retrodiode.com LED illuminator for Zeiss IM microscope. There is a clip at the end of the video which explores an H&E stained heat-fixed slide that Damian prepared (https://youtu.be/dySnojsTteM). In the first video look out for the vortices the organism (I think it is probably Vorticella but needs to be confirmed – see this video for comparison (not ours) https://youtu.be/193EpXPU6QM) is producing either side of its mouth-parts:

x20 objective, live view:

x20 objective, phase contrast Ph1 annulus, live views:

I suspect that the following is a photo of the dead carcass of one of the feeding organisms in the following photos:

x32 objective, live specimen, series of photos of organism feeding – notice how mouth-parts are closed in some photos and open in others. This organism is also seen at the beginning of the video above. In this video you can see the vortices at sides of mouth-parts – especially in phase contrast. I think it is probably Vorticella but needs to be confirmed – see this video for comparison (not ours) https://youtu.be/193EpXPU6QM).

x32 objective, live specimen, phase contrast Ph1 annulus:

x20 objective, H&E stained heat-fixed:

x40 objective, H&E stained, heat fixed:

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