Damian used his renovated Lomo Biolam microscope to view the small organisms we found in our recent trip to Branston water park and cheek cells we collected from his mouth and stained with H&E staining on 2/2/2018.
Andy and Damian
Cheek cells x20 objective
Cheek cells x40 objective
X90 objective. All these images taken afocally from one side of the binoviewer on the Lomo, using hand-held Samsung S7 phone through x10 ocular and objective mentioned. There is also 1.5x multiplication effect from the binoviewer.
Therefore the magnification on image below with x90 objective = 10 x 90 x 1.5 = 1350 x. Contrast and brightness tweaked in the image editor on the Samsung S7 phone – no other processing:
The following photos are all with x10 objective, otherwise as above. They are of organisms from Branston water park:
The following picture shows Damian taking pictures through the ocular using the a ocal imaging technique of holding his camera up to the ocular.
Damian collected this sample 20/2/2018 when Andy & Damian went there. In the previous post, we described how we centrifuged this sample to get it ready for microscopy. In this post, we show microscopic images from the slides we produced.
Bresser Mikrocam 5.0 camera.
Zeiss IM microscope.
Andy & Rhys & Damian
Photo of large ciliated organism x20 objective bright field:
Photos x32 objective – mixture bright field and Phase Contrast images showing bacteria, diatoms, and something I don’t know!
The sample I took from the stream between Netherstowe School in Lichfield and adjacent allottments on 18/2/2018 has been kept in a jar and fed with a spoonful of sugar dissolved in warm water each day (to keep bacteria growing – these are bottom of food chain for larger predatory protozoa). I keep top open to allow oxygen in. The jars were kept next to our french doors to allow light to shine in each day.
Today I used phase contrast on the Zeiss IM microscope to view these images – so images and video below are either in bright field (without phase contrast) or Phase contrast using annulus Ph1 on the Zeiss. All images and video are using x32 long working distance objective and taken with Bresser 5MP Mikrocam camera.
Phase contrast images, x32 objective – show bacteria and a protozoal organism:
Bright field (first) and Phase contrast (Ph1) image (second) of same field to compare:
Video of sample x32 objective – this is brightfield not phase contrast:
We went to Branston Water Park for walk and collected two samples:
(I) Trawl sample collected using homemade plankton net
(II) Sample from around water plants near edge + some plant parts to include surface-living protozoa.
Andy & Damian
Damian uses my homemade Plankton net to collect sample from Branston Water Park lake (I used a filter for an air conditioning system for the net – to give the required pore size which is measured in microns! Those protozoa are very small):
Creating centrifuged pellet from sample from around water plants next to edge lake:
Video of live microscopy of centrigue pellet from Branston Park lake water sample using x32 objective:
I am very proud of this video – definitely one worth watching!! Look out for the two layers of cilia on the one organism and the vortex-producing mouthparts in another clip, sucking in small organisms as food like a Dyson vacuum cleaner!
This first video is a compilation of highlights from the second video that follows afterwards:
Full video – shows a greater range of organisms and behaviour than the highlights video above – if you are having difficulty accessing the embedded video below the URL for direct link to the video on YouTube is https://youtu.be/SKpROQCVp0A)
Photos from live microscopy of centrifuge pellet x32 objective:
I am having some difficulty identifying this. I can get it down to three types of genera but can’t be more specific. Note the two layers of cilia (hair-like projections):
The following looks like algae but is motile – the video shows two of them moving like liners on the sea:
I am not quite sure whether this organism is the same as the ciliated organism above or not. This one is free swimming whereas the other was (I think) attached at one end to some debris:
Is this following the same as above? This one sucked in debris and small organisms like a Dyson vacuum cleaner as I watched. It is on the video but was more impressive at the eyepiece, as the little doomed organisms could more easily be seem being sucked at high speed into the mouthparts (right side of organism in middle of cilia below):
I prepared a brightfield live sample (unstained) of the water I collected from the stream between Netherstowe School in Lichfield and the allotments next door to the school on 18/2/2018 today 20/2/2018 and viewed this using my Zeiss IM microscope (transmitted light).
Bresser Mikrocam 5.0 camera
All following photos & video using x32 long working distance objective – some protozoa and bacteria visible:
This is where the epi-illumination technique comes into its own. The following pictures are of a UK 1£ coin – showing up surface relief differences and tiny scratches that are not otherwise visible to naked eye.
I have used the white balance adjust function in the Bresser MikroCamLabII software (camera control software) to remove the effect of the colour tinge imparted by the mirror in the filter cube on the microscope.
Following photos x4 objective:
Helicon Focus stack – interestingly this does not appear to have improved a great deal on above best focus image: